Catalog Number - 64418-30P-3.9

Nova Similars C18™ is a traditional reversed-phase HPLC stationary phase manufactured on high-purity Type-B spherical silica with a fully endcapped octadecyl (C18) bonded phase. Designed as a cost-effective alternative to Waters™ Nova-Pak® columns, it offers comparable chromatographic behavior for USP L1 methods while providing the strong hydrophobic retention, reproducibility, and broad applicability expected from a conventional C18 stationary phase.

64418-30P-3.9 is a 300 mm x 3.9 mm ID analytical column packed with 4 µm Nova Similars C18 particles. The extended 300 mm length provides maximum resolving power for highly complex sample matrices, difficult impurity profiles, and demanding method development applications. The 4 µm particle size delivers improved efficiency and sharper peak shapes than comparable 5 µm columns while maintaining moderate operating pressure. The 3.9 mm ID format closely matches the dimensions used in many legacy Nova-Pak® methods and typically operates at approximately 0.8–1.5 mL/min.

This USP L1 column is ideal for hydrophobic and neutral compounds requiring strong reversed-phase retention. Common applications include pharmaceutical analysis, impurity profiling, long-chain fatty acid separations, method optimization, and direct replacement of legacy 3.9 mm ID Nova-Pak® C18 columns where method continuity is important.

Key Benefits

• Type-B spherical silica stationary phase

• Suitable alternative for USP L1 methods

• Comparable selectivity to Waters™ Nova-Pak® C18 columns

• Legacy-compatible 3.9 mm ID format

• Maximum resolving power from 300 mm column length

• Higher efficiency than comparable 5 µm columns

• Strong hydrophobic retention

• End-capped C18 bonded phase

Typical Applications

• USP L1 methods

• Reversed-phase HPLC

• Pharmaceutical analysis

• Impurity profiling

• Method development

• Long-chain fatty acid analysis

• Research and development

• Method transfer and validation

Specifications

• Product Type: HPLC Column

• Stationary Phase: Nova Similars C18

• USP Classification: L1

• Particle Size: 4 µm

• Particle Type: Type-B Spherical Silica

• Bonded Phase: C18 (Octadecyl)

• Endcapped: Yes

• Column Dimensions: 3.9 mm ID x 300 mm

• Length: 300 mm

• Recommended Flow Rate: 0.8–1.5 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• 300 mm length provides maximum chromatographic resolution

• 3.9 mm ID format supports legacy Nova-Pak® method replication

• Higher efficiency than comparable 5 µm columns

• Moderate operating pressure on conventional HPLC systems

• Longer run times may be required compared to shorter column formats

• Excellent alternative for many USP L1 methods

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